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Beitragstitel Corneal enzymatic digestion resistance in the presence of estradiol and estradiol plus selective tissue estrogenic activity regulators (STEAR)
Beitragscode P30
Autor:innen
  1. M. Enes Aydemir ELZA Institute Präsentierende:r
  2. Nikki L. Hafezi ELZA Institute
  3. Emilio A. Torres-Netto ELZA Institute
  4. Léonard Kollros ELZA Institute
  5. Mark Hillen ELZA Institute
  6. Farhad Hafezi ELZA Institute
Präsentationsform ePoster
Themengebiete
  • External Disease / Cornea
Abstract-Text Purpose: To determine the impact of estradiol and the selective tissue estrogenic activity regulator (STEAR), tibolone, on corneal resistance to digestion.

Setting: The research was conducted in a controlled laboratory setting at the ELZA Institute, Zurich, Switzerland.

Methods: Freshly prepared ex-vivo porcine corneas (n=48) were divided into three groups. Group A corneas served as untreated controls. Group B corneas were incubated in 20 μmol/l estradiol solution and Group C corneas were incubated in 20 μmol/l estradiol solution and 2.5 mg tibolone prior to digestion in 0.3% collagenase-A solution to assess digestion time until corneal button dissolution.

Results: Group A control corneas showed the strongest resistance to collagenase digestion (31.38 ± 2.03 hours). Corneas from Group B that were pre-conditioned with estradiol showed significantly lower resistance to digestion than Group A control corneas (27.25 ± 1.84 hours, p < 0.01). Corneas from Group C that had been pre-treated with both estradiol and tibolone showed the least resistance to digestion (22.38 ± 2.47 hours), with significant differences to Group B (p < 0.01) and Group A (p < 0.01).

Conclusions: These findings indicate that elevated estradiol levels could significantly affect corneal diseases associated with increased collagenase activity (such as corneal ectasias like keratoconus) or infectious keratitis. Notably, the use of STEAR therapy may further enhance this effect of estradiol.